Characterization of TPNs/ICG-cRGD Nanoparticles

The morphology of TPNs/ICG-cRGD was observed using transmission electron microscopy (TEM) (Titan G2-F20, FEI, USA). The size distribution and ζ potential were measured in ultra-pure water by using a Malvern zeta Sizer Nano series (Nano ZS, Malvern Instruments, UK). The UV-Vis spectra were detected by UV Spectrophotometer (UV-2450, Shimadzu, Japan). The infrared spectra of the nanoparticles were measured using an infrared spectrometer (Alpha, BRUKE, Germany) to confirm the successful conjugation of HS-mPEG or HS-PEG-cRGD. The grafting rate of HS-mPEG or HS-PEG-cRGD was detected by a sulfhydrylation kit [11 (link)]. The entrapment efficiency (EE) and drug loading rate (LR) of ICG were determined by UV Spectrophotometer [22 (link)]. The stability of TPNs/ICG-cRGD in various mediums was evaluated by monitoring changes in nanoparticle size.

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Wang H., Tang C., Xiang Y., Zou C., Hu J., Yang G, & Zhou W. (2024). Tea polyphenol-derived nanomedicine for targeted photothermal thrombolysis and inflammation suppression. Journal of Nanobiotechnology, 22, 146.

Publication 2024

Titan G2-F20 Malvern zeta Sizer Nano series (Nano ZS, UV-2450

Corresponding Organization :

Other organizations : Central South University, Third Xiangya Hospital, Shihezi University

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Variable analysis

independent variables

Morphology of TPNs/ICG-cRGD observed using transmission electron microscopy (TEM)

dependent variables

Size distribution and ζ potential measured in ultra-pure water by using a Malvern zeta Sizer Nano series
UV-Vis spectra detected by UV Spectrophotometer
Infrared spectra of the nanoparticles measured using an infrared spectrometer
Grafting rate of HS-mPEG or HS-PEG-cRGD detected by a sulfhydrylation kit
Entrapment efficiency (EE) and drug loading rate (LR) of ICG determined by UV Spectrophotometer
Stability of TPNs/ICG-cRGD in various mediums evaluated by monitoring changes in nanoparticle size

control variables

Ultra-pure water used for size distribution and ζ potential measurements

positive controls

None specified

negative controls

None specified

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