Five grams of grinded samples either from NS or ES seeds were extracted by ethanol solution 50% (v/v) as described by Gueffai et al. (2022 (link)).
The phenolic compounds that existed in the experimental seeds (NS and ES) were identified using High-Performance Liquid Chromatography (Agilent 1260 infinity HPLC series, USA). The separation of phenolic compounds was carried out as described by Rashid et al. (2021 (link)). The wavelength of the detector was set at 280 nm. 20 µl of the seeds’ ethanolic solution was taken for injection. The column (Akinetex®, Phenomenx, USA), was operated at 30 °C.
The phenolic compounds that existed in the experimental seeds (NS and ES) were identified using High-Performance Liquid Chromatography (Agilent 1260 infinity HPLC series, USA). The separation of phenolic compounds was carried out as described by Rashid et al. (2021 (link)). The wavelength of the detector was set at 280 nm. 20 µl of the seeds’ ethanolic solution was taken for injection. The column (Akinetex®, Phenomenx, USA), was operated at 30 °C.
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