Preparation of whole-cell lysates, SDS-PAGE, and immunoblotting were performed as previously described (15 (link)). Western blots were probed with anti-adiponectin (Millipore, Billerica, MA), anti-β-actin, anti-CCAAT/enhancer binding protein-α (C/EBP-α), anti-perilipin, and anti-PPARγ (Cell Signaling Technology, Beverly, MA) antibodies. Next, blots were probed with horseradish peroxidase-conjugated goat anti-mouse (adiponectin) or anti-rabbit (all other proteins) secondary antibodies (Bio-Rad, Hercules, CA). Relative protein expression was evaluated by densitometry using ImageJ version 1.47 (National Institutes of Health), with β-actin used to control for total protein recovery. RNA isolation, cDNA synthesis, and quantitative real time-PCR (qRT-PCR) were performed as previously described (16 (link)). Relative gene expression was evaluated by the ΔCt method (17 (link)), with β-actin used to control for total mRNA recovery. Primers were designed using Primer-BLAST (National Center for Biotechnology Information) and obtained from Integrated DNA Technologies (Supplemental Table 1).