The depletion
of EVs in FBS was carried out using a methodology proposed earlier.44 (link) Briefly, FBS was ultrafiltered using Amicon
Ultra-15 centrifugal filter devices (100 kDa cutoff, MerkMillipore,
Darmstadt, Germany) for 30 min at 5000g. The filtrate
was collected and measured for particle concentration using NTA. For
this study, the vesicle-depleted conditioned medium was used to isolate
and purify EVs by SEC, and the EV preparations met the optimal quality
as per the guidelines prescribed by the International Society for
Extracellular Vesicles (ISEV).45 (link)
of EVs in FBS was carried out using a methodology proposed earlier.44 (link) Briefly, FBS was ultrafiltered using Amicon
Ultra-15 centrifugal filter devices (100 kDa cutoff, MerkMillipore,
Darmstadt, Germany) for 30 min at 5000g. The filtrate
was collected and measured for particle concentration using NTA. For
this study, the vesicle-depleted conditioned medium was used to isolate
and purify EVs by SEC, and the EV preparations met the optimal quality
as per the guidelines prescribed by the International Society for
Extracellular Vesicles (ISEV).45 (link)
