Quantifying Osteoclast Activity via TRAP

TRAP staining was carried out according to the manufacturer’s instructions (G1492, Solarbio, Beijing, China). The area of the callus was defined as the region of interest. TRAP-positive cells were counted using image analysis software.22 (link)

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Liu H., Tian F., Hu Y., Ping S, & Zhang L. (2023). Liraglutide in Combination with Insulin Has a Superior Therapeutic Effect to Either Alone on Fracture Healing in Diabetic Rats. Diabetes, Metabolic Syndrome and Obesity, 16, 1235-1245.

Publication 2023

G1492

Callus Cells

Corresponding Organization :

Other organizations : Hebei Medical University, North China University of Science and Technology

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Variable analysis

independent variables

TRAP staining

dependent variables

Area of the callus
TRAP-positive cells

control variables

Manufacturer's instructions (G1492, Solarbio, Beijing, China)

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