To measure the expression of surface antigens in MSC-TK(A168H) or MSCs, FACS analysis was performed as described previously (Park et al., 2013 (link)). Briefly, cells were stained for 15 min at 25℃ with fluorochrome-conjugated antibodies against CD29 (Cat. No. 303003; BioLegend, USA), CD90 (Cat. No. 559869; BD Biosciences, USA), CD105 (Cat. No. 323205; BioLegend), CD34 (Cat. No. 343505; BioLegend), CD45 (Cat. No. 304011; BioLegend), HLA-DR (Cat. No. 560896; BD PharmingenTM, USA), and isotype control. The cells were washed with PBS and suspended in flow cytometry staining buffer. Cells were analyzed using an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific) with AttuneTM NxT software.
Protocol detail
Quantifying Surface Antigen Expression in MSC
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Ajou University, Catholic University of Korea
Top 5 similar protocols
Variable analysis
independent variables
- MSC-TK(A168H) cells
- Expression of surface antigens
- Incubation time (15 min)
- Temperature (25°C)
- Fluorochrome-conjugated antibodies against CD29, CD90, CD105, CD34, CD45, HLA-DR
- Isotype control
- Positive control: Not explicitly mentioned
- Negative control: Isotype control
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!