Detecting Epitope-Tagged Proteins in Toxoplasma

The HA epitope was detected with mouse monoclonal antibody (MAb) HA.11 (BioLegend; 901515), rabbit polyclonal antibody (pAb) anti-HA (Invitrogen; PI715500), or mouse MAb HA.11 conjugated to Alexa Fluor-594 (BioLegend 901511). The Ty1 epitope was detected with mouse MAb BB2 (58 (link)). The c-Myc epitope was detected with mouse MAb 9E10 (59 (link)). The V5 epitope was detected with mouse MAb anti-V5 (Invitrogen; R96025). The smOLLAS epitope was detected with rat MAb anti-OLLAS (60 (link)). Toxoplasma-specific antibodies include pAb rabbit anti-IMC6 (49 (link)), mouse MAb anti-ISP1 (23 (link)), pAb rabbit anti-IMC3 (61 (link)), pAb rabbit anti-IMC12 (41 (link)), pAb rabbit anti-ROP13 (62 (link)), MAb mouse anti-F₁β subunit (5F4) (39 (link)), MAb mouse anti-ATrx1 (11G8) (63 (link)), pAb guinea pig anti-NHE3 (64 (link)), MAb mouse anti-MIC2 (65 (link)), MAb mouse anti-ROP7 (66 (link)).

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Back P.S., Moon A.S., Pasquarelli R.R., Bell H.N., Torres J.A., Chen A.L., Sha J., Vashisht A.A., Wohlschlegel J.A, & Bradley P.J. (2023). IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome. mBio, 14(1), e03042-22.

Publication 2023

Alexa Fluor-594 anti-V5

Alexa 594 Anti antibody Antibodies Antibody C myc Epitope Guinea pig Monoclonal antibody Mouse Rabbit Subunit Toxoplasma

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

Antibodies used to detect epitope tags (HA, Ty1, c-Myc, V5, smOLLAS)
Toxoplasma-specific antibodies (anti-IMC6, anti-ISP1, anti-IMC3, anti-IMC12, anti-ROP13, anti-F1β subunit, anti-ATrx1, anti-NHE3, anti-MIC2, anti-ROP7)

dependent variables

Presence and detection of epitope tags (HA, Ty1, c-Myc, V5, smOLLAS)
Presence and detection of Toxoplasma-specific proteins (IMC6, ISP1, IMC3, IMC12, ROP13, F1β subunit, ATrx1, NHE3, MIC2, ROP7)

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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