Hepatoma Cell Culture and Sorafenib Treatment

The HepG2, Huh7, J7 and Hep3B human hepatoma cell lines were routinely cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (HyClone, Road Logan, UT), 100 U/ml penicillin and 100 mg/ml streptomycin at 37 °C with 5% CO2. The hepatoma cells were stimulated with 0–10 μM sorafenib (Sigma–Aldrich, Burlington, MA).

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Tsai H.W., Chen Y.L., Wang C.I., Hsieh C., Lin Y.H., Chu P.M., Wu Y.H., Huang Y.C, & Chen C.Y. (2023). Anterior gradient 2 induces resistance to sorafenib via endoplasmic reticulum stress regulation in hepatocellular carcinoma. Cancer Cell International, 23, 42.

Publication 2023

DMEM sorafenib

Cell lines Cells Cultured medium Eagle Fetal bovine serum Hepatoma Human Penicillin Sorafenib Streptomycin

Corresponding Organization :

Other organizations : National Cheng Kung University Hospital, National Cheng Kung University, China Medical University, Chiayi Chang Gung Memorial Hospital, Chang Gung Memorial Hospital, Chung Shan Medical University

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Variable analysis

independent variables

Sorafenib concentration (0–10 μM)

dependent variables

Cell response to sorafenib treatment

control variables

Cell lines (HepG2, Huh7, J7, and Hep3B human hepatoma cell lines)
Cell culture conditions (Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 mg/ml streptomycin, cultured at 37 °C with 5% CO2)

controls

Positive control: Not specified
Negative control: Not specified

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