Quantitative PCR and Western Blot

Real-time quantitative PCR (qPCR) was performed with corresponding primers (Table S1) in a TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) as described previously (18 (link)).
Western blots were performed as describe before (18 (link)) using primary antibodies specific for S100B (Abcam), full length RAGE (FL-RAGE) (Abcam), β-actin (Santa Cruse), S100A9 (R&D Systems), HMGB1 and GAPDH (Cell Signaling).

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Chen X., Zhang L., Zhang I.Y., Liang J., Wang H., Ouyang M., Wu S., da Fonseca A.C., Weng L., Yamamoto Y., Yamamoto H., Natarajan R, & Badie B. (2014). RAGE Expression in Tumor-associated Macrophages Promotes Angiogenesis in Glioma. Cancer research, 74(24), 7285-7297.

Publication 2014

TaqMan 5700 Sequence Detection System FL-RAGE HMGB1 GAPDH

Actin Antibodies Gapdh Hmgb1 Primers Rage Real time pcr Western blots

Corresponding Organization : City of Hope

Other organizations : Union Hospital, Jilin University, Zhejiang University, Qilu Hospital of Shandong University, Third Xiangya Hospital, Central South University, Universidade Federal do Rio de Janeiro, Kanazawa University

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Variable analysis

independent variables

Primers for real-time quantitative PCR (qPCR)

dependent variables

Expression levels of S100B, full length RAGE (FL-RAGE), S100A9, HMGB1, and GAPDH (measured by Western blots)

control variables

β-actin (used as a reference protein for Western blots)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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