The cross-sectional study was conducted from July to December 2019 and the cohort from January to May 2020. In phase I, CHWs aged 18 years or older were included and those who were on vacation and/or leave on the scheduled dates were excluded. Therefore, of the 172 CHWs, 36 were on vacation or leave and were excluded from the study. The inclusion criteria for the cohort (phase II) were: CHWs who had three doses of the hepatitis B vaccine and anti-HBs titers <10 mIU/mL in the serological test. Individuals in this situation were eligible to receive the challenge dose12.
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