Quantitative MALDI-TOF-MS Imaging of MitoQ

MALDI-TOF-MS imaging is used for label-free bioanalysis of the spatial distribution of pharmaceuticals, biomolecules, and other molecules from a tissue section [26 (link)]. The autoflex speed™ MALDI-TOF-MS imaging (Bruker, Bremen, Germany) was utilized to reveal the quantitative distribution of MitoQ across a tissue section (8 μm) having high spatial resolution from BALB/c nude mice injected with 5 mg/kg/day MitoQ intraperitoneally for three consecutive days. 2,5-Dihydroxybenzoic acid (DHB, Bruker, 8201346, 30 g/L) with 1% trifluoroacetic acid was utilized as the matrix for MALDI-TOF-MS imaging analysis.

Free full text: Click here

Bao X., Liu X., Wu Q., Ye F., Shi Z., Xu D., Zhang J., Dou Z., Huang G., Zhang H, & Sun C. (2023). Mitochondrial-Targeted Antioxidant MitoQ-Mediated Autophagy: A Novel Strategy for Precise Radiation Protection. Antioxidants, 12(2), 453.

Publication 2023

Balb c mice Dihydroxybenzoic acid Maldi ms Mitoq Nude Pharmaceuticals distribution Tissue Trifluoroacetic acid

Corresponding Organization :

Other organizations : Institute of Modern Physics, University of Chinese Academy of Sciences, Ji Hua Laboratory, Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Injection of 5 mg/kg/day MitoQ intraperitoneally for three consecutive days

dependent variables

Quantitative distribution of MitoQ across a tissue section (8 μm) from BALB/c nude mice

control variables

Tissue section (8 μm) from BALB/c nude mice

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!