The homogenate tissue levels of inflammatory mediators, including TNF-α, IL-6, IL-4 and IL-10, were measured using a Milliplex MAP Rat Cytokine/Chemokine magnetic bead immunoassay kit (Millipore Corporation, Billerica, MA)[11 (link)]. The values were read with a MAGPIX Luminex xMAP instrument (Luminex Corp, Austin, TX) and analyzed with the MILLIPLEX Analysis software version 3 (Millipore Corporation, Billerica, MA)[11 (link)]. Blood samples (5 mL) were collected for centrifugation at 3000 rpm for 7 min at low temperature, and then, the supernatants were obtained for the alanine transaminase (ALT) and aspartate transaminase (AST) detection with an Automatic Biochemical Analyzer (AU5400, SIEMENS, Munich, Germany).
Protocol detail
Inflammatory Mediators and Liver Enzymes Quantification
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Milliplex MAP Rat Cytokine/Chemokine magnetic bead immunoassay kit
MILLIPLEX Analysis software version 3
AU5400
Alanine transaminase
Aspartate transaminase
Austin
Blood
Centrifugation
Chemokine
Cytokine
Il 10
Immunoassay
Low temperature
Mediators of inflammatory
Tissue
Tnf α
Corresponding Organization : Chengdu University of Traditional Chinese Medicine
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Variable analysis
independent variables
- None explicitly mentioned
- Homogenate tissue levels of inflammatory mediators (TNF-α, IL-6, IL-4, IL-10)
- Alanine transaminase (ALT) levels
- Aspartate transaminase (AST) levels
- Blood sample collection method (5 mL, centrifugation at 3000 rpm for 7 min at low temperature)
- Automatic Biochemical Analyzer (AU5400, SIEMENS, Munich, Germany) for ALT and AST detection
- Positive control: Not mentioned
- Negative control: Not mentioned
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