Apoptosis Analysis in Multiple Myeloma

Flow cytometry was performed on the human in H929, U266, and 5TGM1 mouse multiple myeloma cells as described previously (32 (link)). Apoptosis was analyzed using Annexin V. Anti-human integrin α_vβ₃ antibody, clone LM609 (Millipore Corporation), anti-mouse integrin α₄ and β₁ (eBiosciences), and rhodamine-labeled NPs were used for flow cytometry (FACScalibur flow cytometer; Becton Dickinson Immunocytometry Systems). Analysis was performed using FLOJO software (Tree Star).

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Soodgupta D., Pan D., Cui G., Senpan A., Yang X., Lu L., Weilbaecher K.N., Prochownik E.V., Lanza G.M, & Tomasson M.H. (2015). Small Molecule MYC Inhibitor Conjugated to Integrin-Targeted Nanoparticles Extends Survival in a Mouse Model of Disseminated Multiple Myeloma. Molecular cancer therapeutics, 14(6), 1286-1294.

Publication 2015

FACScalibur flow cytometer FLOJO software

Annexin v Anti antibody Apoptosis Cells Clone Flow cytometry Human Integrin Integrin αvβ3 Mouse Multiple myeloma Rhodamine Tree

Corresponding Organization : Washington University in St. Louis

Other organizations : University of Illinois Urbana-Champaign, Pittsburg State University, University of Pittsburgh

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Variable analysis

independent variables

Human multiple myeloma cell lines (H929, U266)
Mouse multiple myeloma cell line (5TGM1)

dependent variables

Apoptosis (analyzed using Annexin V)

control variables

Flow cytometry performed as described previously (32)
Anti-human integrin α_v_β_3 antibody, clone LM609 (Millipore Corporation)
Anti-mouse integrin α_4 and β_1 (eBiosciences)
Rhodamine-labeled NPs
FACScalibur flow cytometer (Becton Dickinson Immunocytometry Systems)
Analysis performed using FLOJO software (Tree Star)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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