Corn oil (CO), p-phenylenediamine and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Tamoxifen (TMX) was purchased from Cayman Chemical (Ann Arbor, MI). KU-596, N-(2-(5-(((3R,4S,5R)-3,4-dihydroxy-5-methoxy-6,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-3′-fluoro-[1,1′-bi-phenyl]-2-yl)ethyl)-acetamide, was synthesized as previously described.20 (link) Compound purity routinely exceeded 95%.
c-Jun antibody was purchased from Cell Signaling Technology (#9165, Danvers, MA). Biotinylated Hsp70 antibody was purchased from Enzo Life Sciences (#ADI-SPA-810B, Farmingdale, NY). β-Actin antibody, GAPDH antibody, goat anti-mouse HRP conjugated secondary antibody, and goat anti-rabbit HRP conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Goat anti-rabbit Alexa Fluor-647 conjugated secondary antibody, streptavidin-HRP, and Diamond prolong antifade mounting solution with 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo-Fisher Scientific Inc. (Grand Island, NY).
Tissue-Tek Optimum cutting temperature (OCT) was purchased from Electron Microscopy Sciences (Hatfield, PA). Captisol (CAP) was purchased from Cydex Pharmaceuticals Inc. (Lenexa, KS)
c-Jun antibody was purchased from Cell Signaling Technology (#9165, Danvers, MA). Biotinylated Hsp70 antibody was purchased from Enzo Life Sciences (#ADI-SPA-810B, Farmingdale, NY). β-Actin antibody, GAPDH antibody, goat anti-mouse HRP conjugated secondary antibody, and goat anti-rabbit HRP conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Goat anti-rabbit Alexa Fluor-647 conjugated secondary antibody, streptavidin-HRP, and Diamond prolong antifade mounting solution with 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo-Fisher Scientific Inc. (Grand Island, NY).
Tissue-Tek Optimum cutting temperature (OCT) was purchased from Electron Microscopy Sciences (Hatfield, PA). Captisol (CAP) was purchased from Cydex Pharmaceuticals Inc. (Lenexa, KS)
