The chemicals used in the experiments were of analytical grade and HPLC grade. Trimethylamine hydrochloride (TMA.HCl), Dimethylamine hydrochloride (DMA.HCl), Methylamine hydrochloride (MMA.HCl) were purchased from Himedia and Trimethylamine n-oxide dihydrate from Sigma Aldrich. The syringe-driven lters (MCE hydrophilic, 0.22µm pore size, 13mm diameter) were purchased from Himedia and Eppendorf tubes from Tarsons.