NaAsO2 was used at 200 μM (Sigma). The angiogenin small-molecule inhibitor N65828 (8-amino-5-(4′-hydroxybiphenyl-4-ylazo)naphthalene-2-sulphonate) was obtained from the National Cancer Institute (
Immunofluorescence Assay for Cellular Proteins
NaAsO2 was used at 200 μM (Sigma). The angiogenin small-molecule inhibitor N65828 (8-amino-5-(4′-hydroxybiphenyl-4-ylazo)naphthalene-2-sulphonate) was obtained from the National Cancer Institute (
Corresponding Organization :
Other organizations : University of Cambridge, Wellcome/MRC Cambridge Stem Cell Institute, Medical Research Council, Centre National de la Recherche Scientifique, Johannes Gutenberg University Mainz, Helmholtz Zentrum München, German Center for Lung Research, Ludwig-Maximilians-Universität München, Friedrich Baur Stiftung, University College London, Howard Hughes Medical Institute, Rockefeller University
Protocol cited in 1 other protocol
Variable analysis
- NaAsO2 at 200 μM (Sigma)
- Angiogenin small-molecule inhibitor N65828 (8-amino-5-(4′-hydroxybiphenyl-4-ylazo)naphthalene-2-sulphonate) obtained from the National Cancer Institute
- NAC purchased from Sigma
- O-propargyl-puromycin (OP-puromycin) from Medchem Source LLP
- Expression levels of NSun2, NMP1, angiogenin, eIF4AI, 4ET, SK1, eIF3η, cleaved caspase-3, PSD95, synapsin, GFAP, Tbr1, Dcx
- Concentration of antibodies used for immunodetection
- Source of antibodies
- None specified
- None specified
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!