Vav2 and Vav3 Knockout Mice: Immune System Analysis

Fig. S1 depicts analyses of lymphocyte development in Vav2^ko and Vav3^ko mice. Flow cytometry analyses of thymocytes, splenocytes, LN, and BM cells from WT mice (WT), Vav2^ko (Vav2), or Vav3^ko (Vav3) mice are shown. Fig. S2 shows the strategy for generation of Vav3-deficient mice. Southern blotting analyses of targeted ES cells used to generate chimeric mice and their offspring and Western blotting analyses of Vav3ko splenocyte lysates with Vav3-specific antibodies showing no full-length or truncated Vav3 protein present are also shown. Fig. S3 presents analyses of humoral TD responses in Vav3-deficient mice. Concentrations of TNP-specific IgM, IgG1, IgG2b, and IgG3 antibodies analyzed at various time points post-primary or secondary immunization with TNP-conjugated keyhole hemagglutinin protein (TNP-KLH) are shown. Fig. S4 presents analyses of cytokine production by Vav-deficient T cells showing defective IL-2 and IFNγ production. Figs. S1–4 are available online at http://www.jem.org/cgi/content/full/jem.20030874/DC1.