Preparation of Small Unilamellar Vesicles

Small unilamellar vesicles (SUVs) were prepared as described [58 (link)]. Egg PG and egg PC suspended in chloroform were mixed at an equimolar ratio in a round bottom flask. The chloroform was evaporated under a nitrogen stream and further dried under vacuum for 1 h. Dried lipids were suspended in PBS (10 mM phosphate buffer, 2.7 mM KCl, and 137 mM NaCl, pH 7.4), and 50 nm SUVs were prepared by extruding the suspension through a Whatman membrane. The size of the vesicles was confirmed by dynamic light scattering. Lipid vesicles were stored at 4 °C prior to use.

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Ysselstein D., Dehay B., Costantino I.M., McCabe G.P., Frosch M.P., George J.M., Bezard E, & Rochet J.C. (2017). Endosulfine-alpha inhibits membrane-induced α-synuclein aggregation and protects against α-synuclein neurotoxicity. Acta Neuropathologica Communications, 5, 3.

Publication 2017

Whatman membrane

Buffer Chloroform Lipid Membrane Nacl Nitrogen Phosphate Unilamellar vesicles Vacuum

Corresponding Organization :

Other organizations : Purdue University West Lafayette, Institut des Maladies Neurodégénératives, Massachusetts General Hospital

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Variable analysis

independent variables

Ratio of egg PG and egg PC in the lipid mixture

dependent variables

Size of the vesicles

control variables

Concentration of phosphate buffer (10 mM)
Concentration of KCl (2.7 mM)
Concentration of NaCl (137 mM)
PH of the buffer (7.4)
Extrusion process to produce 50 nm SUVs
Storage temperature of the lipid vesicles (4 °C)

controls

None specified

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