Generation and Characterization of TgF344-AD Rat Model

TgF344-AD rats were generated on a Fischer 344 background by co-injecting rat pronuclei with two human genes driven by the mouse prion promoter: ‘Swedish’ mutant human APP (APP_sw) and Δ exon 9 mutant human presenilin-1 (PS1ΔE9). Both constructs used have previously been reported (Jankowsky et al., 2001 (link)). Transgene integration was confirmed by genotyping and expression levels were evaluated by Western blot (WB) of brain homogenates. Line 19 was selected for colony expansion, aging, and detailed analyses based on highest overall transgene expression levels. TgF344-AD rats and wild-type (WT) littermates were housed and maintained at the Cedars-Sinai Medical Center Department of Comparative Medicine vivarium, and all experiments were conducted in compliance with protocols approved by the Institutional Animal Care and Use Committee. We did not observe gender differences on any of the measures reported, and therefore males and females were combined for all analyses. TgF344-AD rats are available upon request via material transfer agreement.

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Cohen R.M., Rezai-Zadeh K., Weitz T.M., Rentsendorj A., Gate D., Spivak I., Bholat Y., Vasilevko V., Glabe C.G., Breunig J.J., Rakic P., Davtyan H., Agadjanyan M.G., Kepe V., Barrio J.R., Bannykh S., Szekely C.A., Pechnick R.N, & Town T. (2013). A Transgenic Alzheimer Rat with Plaques, Tau Pathology, Behavioral Impairment, Oligomeric Aβ, and Frank Neuronal Loss. The Journal of Neuroscience, 33(15), 6245-6256.

Publication 2013

Brain Exon Females Fischer 344 Human Human presenilin 1 Institutional animal care and use committee Males Medicine Mouse Prion Protocols compliance Rats Transgene Western blot

Corresponding Organization :

Other organizations : Imaging Center, Cedars-Sinai Medical Center, Regenerative Medicine Institute, University of California, Irvine, Yale University, Institute for Molecular Medicine, Neurobehavioral Systems, University of California, Los Angeles

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Variable analysis

independent variables

Transgene integration (confirmed by genotyping)

dependent variables

Transgene expression levels (evaluated by Western blot of brain homogenates)
Behavioral and cognitive measures (not explicitly mentioned)

control variables

Gender (males and females combined for all analyses)
Housing and maintenance conditions (TgF344-AD rats and wild-type (WT) littermates were housed and maintained at the Cedars-Sinai Medical Center Department of Comparative Medicine vivarium)

controls

Positive control: Wild-type (WT) littermates
Negative control: Not explicitly mentioned

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