Quantifying Mouse Cytokines and Chemokines

Mouse cytokines and chemokines were quantitated in grafts and spleens using LEGENDplex panels and analyzed with LEGENDplex Data Analysis Software, V8.0 (Biolegend).^{30 (link)} Cytokine and chemokine levels were depicted as picograms/gram (pg/g) of tissue.

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Dhital R., Anand S., Graber B., Zeng Q., Velazquez V.M., Boddeda S.R., Fitch J.R., Minz R.W., Minz M., Sharma A., Cianciolo R, & Shimamura M. (2022). Murine cytomegalovirus promotes renal allograft inflammation via Th1/17 cells and IL‐17A. American Journal of Transplantation, 22(10), 2306-2322.

Publication 2022

LEGENDplex panels LEGENDplex Data Analysis Software, V8.0

Chemokine Cytokine Grafts Mouse Tissue

Corresponding Organization : The Ohio State University

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Variable analysis

independent variables

Mouse cytokines and chemokines

dependent variables

Cytokine and chemokine levels (picograms/gram of tissue)

control variables

Grafts and spleens

controls

No positive or negative controls were explicitly mentioned.

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