HK-2 Cell Proteome Profiling by LFQMS

Triplicate protein extracts from HK-2 cells, treated with THP or vehicle, were analyzed using LFQMS as previously described (18 (link), 19 (link)). In brief, HK-2 proteins were digested with trypsin and peptides analyzed using a Thermo-Finnigan linear ion-trap (LTQ) mass spectrometer coupled with a Surveyor autosampler and MS HPLC system (Thermo-Finnigan). The data were collected in the “Data dependent MS/MS” mode with the ESI interface and searched against the International Protein Index (IPI) HUMAN database using SEQUEST (v. 28 rev. 12) algorithms in Bioworks (v. 3.3). The searched peptides and proteins were validated by PeptideProphet (64 (link)) and ProteinProphet (65 (link)) in the Trans-Proteomic Pipeline (TPP, v. 3.3.0) (http://tools.proteomecenter.org/software.php). Only proteins with probability ≥ 0.9000 and peptides with probability ≥ 0.8000 were used for quantitation.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

LaFavers K.A., Macedo E., Garimella P.S., Lima C., Khan S., Myslinski J., McClintick J., Witzmann F.A., Winfree S., Phillips C., Hato T., Dagher P., Wu X.R., El-Achkar T.M, & Micanovic R. (2019). Circulating Uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel. Science translational medicine, 11(512), eaaw3639.

Publication 2019

linear ion-trap (LTQ) mass spectrometer Surveyor autosampler MS HPLC system

Cells extracts Hplc Human Ms ms Peptides Proteins Trypsin

Corresponding Organization : New York University

Top 5 similar protocols

Variable analysis

Dependent Variables not detected.
Unable to provide accurate variables.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!