Colorimetric Caspase-3 Activity Assay

Caspase-3 activity was analyzed using A172 cell lysates with a colorimetric protease assay (Thermo Fisher Scientific, Inc.) as previously described (37 (link)). The absorbance was read using a microplate reader (Synergy 2-BioTek; Agilent Technologies, Inc.) at 400 nm.

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Genovese C., Cambria M.T., D'angeli F., Addamo A.P., Malfa G.A., Siracusa L., Pulvirenti L., Anfuso C.D., Lupo G, & Salmeri M. (2020). The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. International Journal of Oncology, 57(5), 1129-1144.

Publication 2020

colorimetric protease assay Synergy 2-BioTek

Assay Caspase 3 Cell Colorimetric Protease

Corresponding Organization :

Other organizations : University of Catania, Istituto di Chimica Biomolecolare

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Caspase-3 activity

control variables

A172 cell lysates

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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