Cresyl violet (CV) stains the endoplasmic reticulum and the cellular nuclei in dark purple, which includes, in the brain, cell bodies of neurons and glial cells (Eilam-Altstädter et al., 2021) (link). The protocol followed guidelines of US Army Inst Pathol (Luna et al., 1968) , in which the sections were hydrated first, submerged for 1h15 in 0.01% Cresyl violet (Sigma Aldrich catalog #C5042) in a 0.1M acetate buffer (Anachemia, AC-8218) and 0.1M acetic acid (BDH 10001CU), and then dehydrated.
Protocol detail
Cresyl Violet Neuronal Staining
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Université du Québec à Trois-Rivières
Other organizations : Douglas Mental Health University Institute, McGill University, Montreal Neurological Institute and Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Cresyl violet (CV) staining
- Staining of the endoplasmic reticulum and cellular nuclei in dark purple
- Staining of cell bodies of neurons and glial cells in the brain
- Hydration and dehydration of the sections
- Submersion time in 0.01% Cresyl violet solution
- Acetate buffer (0.1M) and acetic acid (0.1M) composition
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!