Concentrated sulfuric acid, Cyclohexane, 37% Formaldehyde solution, Concentrated ammonia, Diethylamine, Sodium carbonate, Sodium nitroprusside purchased from Fisher, UK, Acetaldehyde, Diphenylamine, anhydroussodium sulphate (Alpha chemika, India), Chloroform (Loba Chemie, India), Ethyl acetate (Sigma Aldrich, US), Methanol (AR) purchased from Sigma Aldrich, Chloroform (AR) were purchased from VWR, UK used for analysis process.