CHCHD2 and HIF-1α Protein Expression

The expression of CHCHD2 and HIF-1α protein in all the tissue sections of the subjects were investigated by rabbit antibodies (anti-CHCHD2, diluted 1:50, ABGENT, SanDiego, CA or anti-HIF-1α, diluted 1:100, Proteintech™, Chicago, USA). The detailed operation process and staining score of immunohistochemistry accordance with our previous methods [18 (link)]. The final immunohistochemical staining score reported is the average of the scores from the tow investigators.

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Yin X., Xia J., Sun Y, & Zhang Z. (2020). CHCHD2 is a potential prognostic factor for NSCLC and is associated with HIF-1a expression. BMC Pulmonary Medicine, 20, 40.

Publication 2020

anti-HIF-1α

Antibodies Protein Rabbit Tissue

Corresponding Organization : Air Force Medical University

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Variable analysis

independent variables

Expression of CHCHD2 protein
Expression of HIF-1α protein

dependent variables

Immunohistochemical staining score

control variables

Tissue sections of the subjects
Rabbit antibodies (anti-CHCHD2, diluted 1:50, ABGENT, SanDiego, CA or anti-HIF-1α, diluted 1:100, Proteintech™, Chicago, USA)
Immunohistochemistry procedures and staining score according to previous methods [18]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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