To observe the central element we used an antibody (Novus, NB300-229) raised against the central element protein; Synaptonemal Complex Protein 1 (SYCP1). We employed the use of an SMC3 antibody (Abcam, Ab9263) to visualise the axial core in platypus since it has been previously shown that SMC3 recruitment follows synaptic progression39 (link).
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