Immunohistochemical Analysis of NR5A2, GDF15, and Ki67

Tissue microarray was purchased from Outdo Biobank (Shanghai, China) (HPan-Ade060CD-01). IHC analysis was performed with NR5A2 (#22460-1-AP, working dilution 1:5000, Proteintech), GDF15 (#27455-1-AP, working dilution 1:500, Proteintech), Ki67(27309-1-AP, working dilution 1:3000, Proteintech) antibodies. Two independent pathologists, who were uninformed about the patient data and histopathological features of the samples, were responsible for reviewing and scoring the degree of immunostaining separately. Staining intensity scoring was performed as described previously^{43 (link)}.

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Guo F., Zhou Y., Guo H., Ren D., Jin X, & Wu H. (2021). NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15. Cell Death Discovery, 7, 78.

Publication 2021

Ki67(27309-1-AP

Antibodies Dilution Gdf15 Microarray Pathologists Patient Tissue

Corresponding Organization : Central South University

Other organizations : Union Hospital, Huazhong University of Science and Technology, Union Hospital

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Variable analysis

independent variables

Tissue microarray purchased from Outdo Biobank (Shanghai, China) (HPan-Ade060CD-01)

dependent variables

Degree of immunostaining for NR5A2, GDF15, and Ki67 as measured by IHC analysis

control variables

Two independent pathologists, who were uninformed about the patient data and histopathological features of the samples, were responsible for reviewing and scoring the degree of immunostaining separately

controls

Staining intensity scoring was performed as described previously^{43 (link)}

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