Curcumin Treatment Impacts Cell Proliferation

The pRc/RSV-derived eukaryotic expression vectors pRc-HA-v-Myc and pRc-v-Src have been described (34 (link)). Transcriptional transactivation analysis using the luciferase reporter system, including the firefly luciferase constructs pGL3-WS5 (pLUC-WS5) and the empty vector pGL3-Basic (pLUC), has been described (33 (link), 34 (link), 36 (link)). The eukaryotic expression construct pcDNA3.1-Rluc used for constitutive renilla luciferase expression, and the empty pcDNA3.1 vector have been described (34 (link)). Proliferation of cells treated with curcumin (CRM) was monitored in real time by using the live cell imaging system IncuCyte S3 (Essen Bioscience/Sartorius, Vienna, Austria) as described (33 (link)). Cells were seeded in a 24-well dish (Corning, Vienna, Austria), and incubated overnight. Curcumin was then added to final concentrations of 5–40 µM. Cells were monitored for 3 d by phase contrast imaging every 6 h from 16 separate regions per well using a 10× objective.

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Mödlhammer A., Pfurtscheller S., Feichtner A., Hartl M, & Schneider R. (2021). The Diarylheptanoid Curcumin Induces MYC Inhibition and Cross-Links This Oncoprotein to the Coactivator TRRAP. Frontiers in Oncology, 11, 660481.

Publication 2021

24-well dish

Cells Cells proliferation Curcumin Dish Eukaryotic Firefly luciferase Luciferase Pgl3 Phase contrast Renilla luciferase Transactivation Transcriptional V myc V src Vector

Corresponding Organization :

Other organizations : Universität Innsbruck

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Variable analysis

independent variables

Curcumin (CRM) concentration (5-40 µM)

dependent variables

Proliferation of cells

control variables

Cells seeded in a 24-well dish and incubated overnight

controls

Empty vector pGL3-Basic (pLUC)
Constitutive renilla luciferase expression construct pcDNA3.1-Rluc

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