For the disk diffusion test, E. faecalis at a concentration of 108 CFU/mL in 100 uL suspension and C. albicans at a concentration of 106 CFU/mL in 100 uL suspension were seeded on Mueller Hinton agar plates (Biomerieux, Lyon, France) [58 (link),59 (link)].
Seven different study groups containing calcium hydroxide and Cyprus mountain thyme oil (O. dubium EO) or garden mint oil (M. spicata EO) were tested for antimicrobial activity. For this purpose, the different groups formed were added to the sterile disks to be tested. Distilled water was chosen as a negative control for both microorganisms. Vancomycin was chosen as a positive control for E. faecalis and nystatin for C. albicans.
Agar plates for E. faecalis were incubated at 37 °C for 24, 48, and 72 h. C. albicans was incubated at 37 °C for 48 and 72 h. The inhibition zones formed around the disks on the agar plates indicated the degree of antimicrobial activity (Figure 4 ). Inhibition zone measurements (mm) were taken at 24, 48, and 72 h for E. faecalis and 48 and 72 h for C. albicans. Each test was repeated in triplicate to obtain reliable data.
Seven different study groups containing calcium hydroxide and Cyprus mountain thyme oil (O. dubium EO) or garden mint oil (M. spicata EO) were tested for antimicrobial activity. For this purpose, the different groups formed were added to the sterile disks to be tested. Distilled water was chosen as a negative control for both microorganisms. Vancomycin was chosen as a positive control for E. faecalis and nystatin for C. albicans.
Agar plates for E. faecalis were incubated at 37 °C for 24, 48, and 72 h. C. albicans was incubated at 37 °C for 48 and 72 h. The inhibition zones formed around the disks on the agar plates indicated the degree of antimicrobial activity (
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