Multilocus Sequence Typing of C. jejuni

A total of 341 C. jejuni strains of known Multilocus sequence types (MLST) were included in this study. The strain collection was composed of 41 strains isolated from dairy cattle at farm level, 98 strains isolated from retail broiler products, 101 strains isolated from wild birds and 101 strains from human clinical cases that were collected at the Microbiological Laboratory of Kaunas Clinical Hospital over one year period. The MLST method based on sequencing of seven housekeeping genes was applied for each of the isolates as described in a previous study (Ramonaite et al., 2014 (link), 2017 (link)). The strains were stored at -80°C in brain heart infusion broth (BHI) (Oxoid, Basingstoke, United Kingdom) with 30 % glycerol (Stanlab, Lublin, Poland).

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Aksomaitiene J., Ramonaite S., Tamuleviciene E., Novoslavskij A., Alter T, & Malakauskas M. (2019). Overlap of Antibiotic Resistant Campylobacter jejuni MLST Genotypes Isolated From Humans, Broiler Products, Dairy Cattle and Wild Birds in Lithuania. Frontiers in Microbiology, 10, 1377.

Publication 2019

glycerol

Birds Brain Cattle Glycerol Heart Housekeeping genes Human Strains

Corresponding Organization : Lithuanian University of Health Sciences

Other organizations : Freie Universität Berlin

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Variable analysis

independent variables

Strain source: dairy cattle, retail broiler products, wild birds, human clinical cases

dependent variables

Multilocus sequence types (MLST) of Campylobacter jejuni strains

control variables

Storage conditions of the C. jejuni strains: -80°C in brain heart infusion broth with 30% glycerol

controls

No positive or negative controls were explicitly mentioned in the input.

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