Primary antibodies used in this study include rat anti-HA (Roche clone 3F10), VVL-FITC (1:1,000, Vector FL1231); DMC1 antibody (1:10, a kind gift of Christopher Huston, University of Vermont [15 (link)]), rabbit anti-TrpB (31 (link)) and mouse anti-alpha tubulin (a kind gift of Jacek Gaertig, University of Georgia), both used at 1:300; and secondary antibodies carrying a variety of Alexa Fluors (Abcam) at 1:1,000.
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