Microarray Analysis of CCOC Cell Lines

RNA of 12 human CCOC cell lines, 10 human CCOC specimen and 10 normal ovarian surface epithelium specimen were extracted and microarray analysis was performed as described protocol [12 (link)]. In brief, following a single strand reverse transcriptase and amplification step, samples were hybridized to Affymetrix U133 Plus 2.0 microarrays. Class comparison in BRB ArrayTools version 3.2.2 software was utilized to identify differentially expressed genes.

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Tsang T.Y., Wei W., Itamochi H., Tambouret R, & Birrer M.J. (2017). Integrated genomic analysis of clear cell ovarian cancers identified PRKCI as a potential therapeutic target. Oncotarget, 8(57), 96482-96495.

Publication 2017

U133 Plus 2.0 microarrays

Cell lines Epithelium Genes Human Microarrays Ovarian Reverse transcriptase

Corresponding Organization : Harvard University

Other organizations : Tottori University

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Variable analysis

independent variables

Cell lines: 12 human CCOC cell lines
Specimens: 10 human CCOC specimens
Specimens: 10 normal ovarian surface epithelium specimens

dependent variables

Gene expression profiles from microarray analysis

control variables

RNA extraction and microarray analysis protocol [12] as described

controls

No positive or negative controls were explicitly mentioned in the provided information.

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