Plant height of Huaai 11 and other four agronomic traits were evaluated for seven years at two locations (Yaan, Sichuan Province, and Wuhan, Hubei Province). The results showed that dwarfism is constantly expressed with height of 40.8 ± 1.84 cm (Table 1 ). Its maturity is about 7 days earlier than the control Zaoshu 3 (early maturity variety).
For genetic analysis, Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, and Advance. The 5 F2 populations, their F1 and parents were planted in field in 1998. Additional 4 tall varieties, Huadamai 1, Huadamai 6, Hyproly and Ris01508 were crossed with Huaai 11 in year 2000. The 9 F2 populations, their F1 and parents were planted in 2002 (Table2 ).
To test the allelic relationship of Huaai 11 with other dwarfing genes in barley, The F1 of Huaai 11 × Monker that is a popular commercial cultivar developed in USA was crossed with four dwarf varieties, Himalaya (br, 65.7 cm), Aiganqi (uzu,71.7 cm), India dwarf (sdw1, 61.3 cm) and Maris Mink (denso, 69.2 cm).
All abovementioned generations and their parents were grown on the Experimental Farm of Huazhong Agricultural University, Wuhan. A randomized complete block design with three replications was adopted. The materials were planted in five-row plots with a row length of 1.1 m.
To map the dwarfism gene in Huaai 11, a population consisting of 122 doubled-haploid (DH) lines was developed from a cross between a common feed barley cultivar Huadamai 6 and Huaai 11 using anther culture in this study. The DH population and parents were planted on the Experimental Farm of Huazhong Agricultural University, Wuhan, China. The field trials were conducted following a randomized complete block design with three replications in 2006, 2007 and 2008, respectively. Each of the DH and parental lines were grown in three rows in a plot of 0.6 × 1.5 m2.
Height of plants before ripening was measured in the field from soil surface to top of the main culm (with the spike). The height was calculated as the mean of the twelve plants (in three replicates, four plants from each replicate were measured).
For genetic analysis, Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, and Advance. The 5 F2 populations, their F1 and parents were planted in field in 1998. Additional 4 tall varieties, Huadamai 1, Huadamai 6, Hyproly and Ris01508 were crossed with Huaai 11 in year 2000. The 9 F2 populations, their F1 and parents were planted in 2002 (Table
To test the allelic relationship of Huaai 11 with other dwarfing genes in barley, The F1 of Huaai 11 × Monker that is a popular commercial cultivar developed in USA was crossed with four dwarf varieties, Himalaya (br, 65.7 cm), Aiganqi (uzu,71.7 cm), India dwarf (sdw1, 61.3 cm) and Maris Mink (denso, 69.2 cm).
All abovementioned generations and their parents were grown on the Experimental Farm of Huazhong Agricultural University, Wuhan. A randomized complete block design with three replications was adopted. The materials were planted in five-row plots with a row length of 1.1 m.
To map the dwarfism gene in Huaai 11, a population consisting of 122 doubled-haploid (DH) lines was developed from a cross between a common feed barley cultivar Huadamai 6 and Huaai 11 using anther culture in this study. The DH population and parents were planted on the Experimental Farm of Huazhong Agricultural University, Wuhan, China. The field trials were conducted following a randomized complete block design with three replications in 2006, 2007 and 2008, respectively. Each of the DH and parental lines were grown in three rows in a plot of 0.6 × 1.5 m2.
Height of plants before ripening was measured in the field from soil surface to top of the main culm (with the spike). The height was calculated as the mean of the twelve plants (in three replicates, four plants from each replicate were measured).
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