The F-actin assembling was assessed as previously described (Ge et al., 2016 (link)), samples were incubated in Alexa Fluor 488 conjugated phalloidin reagents (Life Technologies, Waltham, MA, United States) at room temperature for 30 min. Images were visualized with a confocal microscope (Carl Zeiss, Weimar, Germany) and measured using Zen 2011 software (Carl Zeiss, Weimar, Germany).
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