Visual acuity threshold was measured daily by optokinetic tracking (OKT) response using Optometry software and apparatus (CerebralMechanics Inc., Medicine Hat, AB T1A 5K6, Canada), as previously described [8 (link),9 (link),11 (link)]. Briefly, the mice were surrounded by a virtual cylinder consisting of vertical lines rotating at varying frequencies, and tracking behavior was assessed. Visual acuity is represented as the highest spatial frequency at which the mice track the rotating cylinder. Because optokinetic tracking is a temporal-to-nasal reflex, counter-clockwise and clockwise rotations exclusively test the right and left eyes, respectively. Occasionally, EAE-induced motor deficits left mice without the balance and stability to adequately perform OKT testing. In these instances, a measurement was not recorded for the affected mouse on that day.
Protocol detail
Optokinetic Tracking for Visual Acuity
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Corresponding Organization :
Other organizations : Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center
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Variable analysis
independent variables
- Rotating frequency of the virtual cylinder
- Visual acuity threshold
- Vertical lines in the virtual cylinder
- Clockwise and counter-clockwise rotations of the virtual cylinder
- None mentioned
- None mentioned
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