Apoptosis Detection in NTP-Treated Cells

Quantitative apoptotic cell death by plasma was detected using the Annexin V-propidium iodide (PI) apoptosis detection kit I according to the manufacturer's protocol (BD Biosciences, Bedford, MA, USA), as described previously.12 (link) The cells were treated with various gas device (Ar, N₂, and He) NTP for 1, 3, and 5 min and then incubated further for 24 h. The cells were harvested, washed with cold phosphate-buffered saline (PBS), and stained with Annexin V-fluorescein isothiocyanate and PI at room temperature for 15 mins in the dark. The early and late apoptosis were quantified according to the manufacturer's instructions. Apoptosis was detected using a FACS Aria system (BD Biosciences), with the excitation and emission settings of 488 and 530 nm, respectively.