Precision-cut ectopic lung slices (275 μm) were prepared25 (link). After washes to remove residual agarose, slices were placed onto cell culture inserts (Millicell) within 35-mm dishes containing 1 ml recording medium, sealed with a coverslip and placed under a self-contained Olympus LV200 luminescence microscopy system fitted with a cooled Hamamatsu C9100-13 EM-CCD camera (Olympus, Japan) as previously described27 (link). Individual regions of interest were delineated using ImageJ software (v1.41o).
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Precision-cut Ectopic Lung Slice Imaging
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Other organizations : Manchester Academic Health Science Centre, University of Manchester, University of Pennsylvania, Philadelphia University, Children's Hospital of Philadelphia, Centre for Inflammation Research, Baylor College of Medicine
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Variable analysis
independent variables
- Precision-cut ectopic lung slices (275 μm)
- Luminescence/light emission from the lung slices
- Washes to remove residual agarose
- Lung slices placed onto cell culture inserts (Millicell) within 35-mm dishes containing 1 ml recording medium
- Lung slices sealed with a coverslip
- Olympus LV200 luminescence microscopy system fitted with a cooled Hamamatsu C9100-13 EM-CCD camera (Olympus, Japan)
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