Western blot analysis was carried out as previously described [12 (link), 31 (link), 32 (link)]. Primary antibodies used are as follows: HSP90, Gapdh (Ambion), γH2AX, GATA3, CtIP (Cell Signaling). For qRT-PCR, total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer’s protocol and cDNA was generated using the RT Kit (YEASEN, Shanghai, China). qRT-PCR was performed as previously reported [35 (link)].
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