For gain-of-function experiments, we used the pMXs retroviral expression system as previously described [2 (link)]. Retroviral vectors expressing deletion mutant of NFIA were constructed using the KOD mutagenesis kit (TOYOBO) according to the manufacturer’s instructions. A retroviral vector expressing Cre recombinase was a gift from Kai Ge (Addgene plasmid # 34564).
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