Protein Extraction and Western Blotting

Protein extraction and western blotting experiments were performed as already described^{61 (link),62}. The antibodies used were: anti-β-actin (sc-1616, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), anti-γ-tubulin (sc-17787, Santa Cruz), anti-GAPDH (sc-32233, Santa Cruz), anti-vinculin (sc-7649, Santa Cruz), anti-PAX8^{63 (link)}, anti-NKX2.1^{33 (link)}, anti-FOXE1^{64 (link)}, anti-SP-B (ab3430, Millipore), anti-SP-C (90716, Abcam), anti-HIPK2 (Novus Biologicals), anti-HMGA1^{65 (link)}. Densitometric analyses of western blot were performed with Image J software.

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Gerlini R., Amendola E., Conte A., Valente V., Tornincasa M., Credendino S.C., Cammarota F., Gentile C., Di Guida L., Paladino S., De Vita G., Fusco A, & Pierantoni G.M. (2019). Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death & Disease, 10(10), 747.

Publication 2019

anti-β-actin anti-γ-tubulin anti-GAPDH anti-vinculin anti-SP-C

Actin Antibodies Biologicals Densitometric Gapdh Novus Protein Tubulin Vinculin Western blot analyses

Corresponding Organization : Institute for Experimental Endocrinology and Oncology

Other organizations : University of Naples Federico II

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Variable analysis

independent variables

Protein extraction and western blotting experiments

dependent variables

Densitometric analyses of western blot

control variables

β-actin
γ-tubulin
GAPDH
Vinculin

controls

Positive controls: anti-PAX8, anti-NKX2.1, anti-FOXE1, anti-SP-B, anti-SP-C, anti-HIPK2, anti-HMGA1
Negative controls: Not explicitly mentioned

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