Serum Corticosterone Response to Restraint

To measure changes in serum levels of corticosterone in response to physical restraint, blood samples (∼300 μL) were collected using the “tail clip” method (Vahl et al., 2005 (link)) before (at rest) and at 10, 60, and 120 min after the restraint for 30 min as previously described by our laboratory (Ju et al., 2017 (link)). Physical restraint was administered using rodent holders (Kent Scientific Corporation, Torrington, CT, United States). Serum corticosterone was measured using commercial ELISA kits (Cayman Chemical Company, Ann Arbor, MI, United States) according to the manufacturer’s instructions.

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Wang J., Yang B., Ju L., Yang J., Allen A., Zhang J, & Martynyuk A.E. (2020). The Estradiol Synthesis Inhibitor Formestane Diminishes the Ability of Sevoflurane to Induce Neurodevelopmental Abnormalities in Male Rats. Frontiers in Systems Neuroscience, 14, 546531.

Publication 2020

rodent holders

Blood Cayman Clip Corticosterone Elisa Physical restraint Rodent Serum Tail

Corresponding Organization : Zhengzhou University

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Variable analysis

independent variables

Physical restraint

dependent variables

Serum corticosterone levels

control variables

Time points for blood collection (at rest, 10 min, 60 min, 120 min after restraint)
Blood sample volume (∼300 μL)
Measurement method (ELISA kits)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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