Microbial Functional Profiling from Sequencing

In this experiment, independent samples Tukey test was performed using SPSS 25.0 statistical software. p < 0.05 indicated that the difference was statistically significant. Other data were processed using mean ± standard deviation, and OriginPro 2012 was used for plotting. KneadData software was used for quality control (based on Trimmomatic) and host removal (based on Bowtie2) of the raw data. Before and after KneadData, FastQC was used to check the rationality and effect of quality control [32 (link),33 (link)]. Starting from the quality-controlled reads and the reads with host genes removed, HUMAnN3 software (based on DIAMOND) was used to align the reads of each sample to the database (UniRef90). According to the UniRef90 ID and the corresponding relationship of each database, the annotation information and relative abundance table of each functional database were obtained [34 (link),35 (link),36 (link),37 (link)]. The original data obtained from liquid chromatography-mass spectrometry (LC-MS/MS) was processed using Proteowizard software (v3.0.8789).

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Shu S., Jing R., Li L., Wang W., Zhang J., Luo Z., Shan Y, & Liu Z. (2024). Effects of Different Heat Treatments on Yak Milk Proteins on Intestinal Microbiota and Metabolism. Foods, 13(2), 192.

Publication 2024

SPSS 25.0 statistical

Corresponding Organization : Tibet University

Other organizations : Shanghai Academy of Agricultural Sciences

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