Liver samples from the experimental and control groups were processed, cleaned, and placed in 0.9% (w/v) NaCl physiological solution for 30 min, then fixed in 10% formaldehyde solution for 24 h. The processed tissues were dehydrated in increasing concentrations of 70%, 80%, 90%, 96% absolute alcohol, and finally with xylol for 1 h for the substitution of alcohol by xylol. Then the tissues were submerged in molten paraffin (62 °C) for the xylol to come out of the tissue and be replaced by paraffin. Finally, cubic molds containing the tissue were filled with paraffin, and after cooling, they were cut with the aid of a sliding microtome (rotary hand processor STP 120, Thermo Scientific MICROM, International GmbH (Robert-Bosch-Str. 49 69,190 Walldorf/Germany) in sections in serial layers with a thickness of 4 mm. These were extended by floating on the surface of warm water. Subsequently, the slides were immersed in descending concentrations of xylol and alcohol for tissue staining by the hematoxylin method and eosin (H and E). This process was performed in triplicate to obtain adequate image resolution of the tissue. Finally, examination was conducted through the light electron microscope at 20X [11 (link),23 (link),38 (link)].
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