Mahonia aquifolium Extracts Phytochemical Analysis

Fresh Mahonia aquifolium (Pursh) Nutt. bark, leaves, flowers, and fruits were purchased from the A. Borza Botanical Garden “Babes-Bolyai” University of Cluj-Napoca, Romania, between April and June 2018 and extracted in the Mycology Laboratory of “Babes-Bolyai” University, Cluj-Napoca, Romania, as previously described by a modified Squibb repercolation method with 70% ethanol (Merck, Bucuresti, Romania), producing the following extracts of M. aquifolium: bark extract 1 : 1.5 (g : mL) (1), leaf extract 1 : 1.2 (g : mL) (2), flower extract 1 : 1 (g : mL) (3), green fruit extract 1 : 1 (g : mL) (4), and ripe fruit extract 1 : 1 (g : mL) (5). The phytochemical analysis of the extracts has been performed and previously published [26 (link), 30 ]. The plants were taxonomically identified and authenticated, and voucher specimens (number 665978) were deposited in “Alexandru Borza” Botanical Garden Herbarium, “Babes-Bolyai” University of Cluj-Napoca, Romania. The stock solutions were diluted with Phosphate-Buffered Saline Solution (PBS, from Sigma-Aldrich Company, St. Louis, USA), to obtain for each extract a series of stock concentrations from 500 to 10 μg plant/mL.