Total RNA was extracted using the mirVana PARIS RNA kit, according to the manufacturer instructions (Life Technologies, California, USA) using 500 μL of serum. After the denaturation, 25 fmol of synthetic miRNA cel-miR-23843 (link) (where “cel” corresponds to the abbreviation of species Caenorhabditis elegans) was added, which was used as quality control of RNA extraction and RT-qPCR.
Free full text: Click here