Destabilized ATP Aptamer Purification

The “destabilized” ATP aptamer (5′- CTGGGGGAGTATTGCGGAGGAAA-3′) oligonucleotide sequence^{24 (link)} was purified using dual HPLC (Biosearch Technologies, CA) and used as received. Tris-2-carboxyethyl-phosphine (TCEP), ferrocenemethanol (FcMeOH), 6-mercapto-1-hexanol (99%), Trizma (tris) base (2-amino- 2-(hydroxymethyl)-1,3-propanediol, magnesium chloride (MgCl₂), sulfuric acid (H₂SO₄), potassium chloride (KCl), hydrogen peroxide (H₂O₂), hydrochloric acid (HCl) sodium chloride (NaCl), adenosine triphosphate (ATP), dopamine hydrochloride (99%) (Alfa Aesar), dimethyl sulfoxide (DMSO), and 10× Tris-EDTA were used as received from Sigma- Aldrich. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25°C) using a Biopak Polisher Millipore ultra purification system (Millipore, Billerica, MA).

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Santos-Cancel M., Simpson L.W., Leach J.B, & White R.J. (2019). Direct, Real-Time Detection of Adenosine Triphosphate Release from Astrocytes in 3-Dimensional Culture Using an Integrated Electrochemical, Aptamer-Based Sensor. ACS chemical neuroscience, 10(4), 2070-2079.

Publication 2019

dual HPLC Tris-2-carboxyethyl-phosphine magnesium chloride sulfuric acid hydrogen peroxide adenosine triphosphate (ATP), dopamine hydrochloride 10× Tris-EDTA Biopak Polisher Millipore ultra purification system

6 mercapto 1 hexanol Adenosine triphosphate Dimethyl sulfoxide Dopamine hydrochloride Edta Ferrocenemethanol H2o2 Hplc Hydrochloric acid Magnesium chloride Nacl Oligonucleotide Propanediol Sulfuric acid Tris Tris 2 carboxyethyl phosphine Trizma

Corresponding Organization : University of Cincinnati

Other organizations : University of Maryland, Baltimore County

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Variable analysis

independent variables

Destabilized ATP aptamer oligonucleotide sequence

dependent variables

Not explicitly mentioned

control variables

FcMeOH
6-mercapto-1-hexanol
Trizma (tris) base
Magnesium chloride (MgCl2)
Sulfuric acid (H2SO4)
Potassium chloride (KCl)
Hydrogen peroxide (H2O2)
Hydrochloric acid (HCl)
Sodium chloride (NaCl)
Adenosine triphosphate (ATP)
Dopamine hydrochloride
Dimethyl sulfoxide (DMSO)
10× Tris-EDTA

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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