The developmental process of inflorescence and floral organogenesis were observed under a stereomicroscope and a scanning electron microscope. The selected plants described above (collected at 5, 10, 15, 18, 21, 24, 27, 30, and 33 DAG) were dissected using a Leica S6D stereomicroscope and fixed in FAA (37% formaldehyde/glacial acetic acid/95% ethanol/dH2O, 10:5:50:35, v/v) at least over night at room temperature [51 ]. Samples were then dehydrated for 15 min in each of the following concentrations 50%, 70%, and 90% ethanol (1×/ea.) and 100% ethanol (3×) at room temperature [34 (link)]. Critical point drying was obtained (Samdri-790, Tousimis Research Corporation, Rockville, MD, USA) mounted on aluminum stubs and platinum coated. One hundred and five plants were sampled, and their inflorescences observed and imaged using scanning electron microscopy (Hitachi S-3500N, Tokyo, Japan) under high vacuum.
To evaluate the timing of inflorescence development and floral organogenesis, a total of 98 plants were evaluated considering the developmental stage of the oldest inflorescence in the plant. The developmental stage was assigned according to the nine stages proposed herein for inflorescence development in TK.
To evaluate the timing of inflorescence development and floral organogenesis, a total of 98 plants were evaluated considering the developmental stage of the oldest inflorescence in the plant. The developmental stage was assigned according to the nine stages proposed herein for inflorescence development in TK.
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