Radiosensitizer and Cell Cycle Inhibitor Effects

Irradiation as well as treatments with erlotinib and cetuximab were described previously (6 (link)). NU7026 (EMD Millipore, 260961), hesperadin (Selleck, S1529), Gö6976 (LC Laboratories, G-6203), and AZD6244 (Selleck, S1008) were dissolved in dimethyl sulfoxide (DMSO) and chloroquine (Sigma, C6628) was dissolved in double distilled water. Drugs were added 1 hour prior to irradiation and maintained for the duration of each experiment. Chaetocin (Cayman, CAS 28097) and Ro-32-0432 (Merck, 557525) were dissolved in DMSO and incubated with cells for 1 hour before collecting for cell lysates. Thymidine (Sigma, T1895) was dissolved in PBS, and used at 2.5 mM concentration 16 hours before and 8 hours after releasing cells in normal cell culture medium.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Wang M., Kern A.M., Hülskötter M., Greninger P., Singh A., Pan Y., Chowdhury D., Krause M., Baumann M., Benes C.H., Efstathiou J.A., Settleman J, & Willers H. (2014). EGFR-Mediated Chromatin Condensation Protects KRAS-Mutant Cancer Cells Against Ionizing Radiation. Cancer research, 74(10), 2825-2834.

Publication 2014

NU7026 hesperadin Gö6976 AZD6244 chloroquine Chaetocin Ro-32-0432 Thymidine

Azd6244 Cayman Cells Cetuximab Chaetocin Chloroquine Dimethyl sulfoxide Drugs Erlotinib Hesperadin Irradiation Normal cell culture Nu7026 Ro 32 0432 Thymidine

Corresponding Organization :

Other organizations : University Hospital Carl Gustav Carus, Helmholtz-Zentrum Dresden-Rossendorf, Dana-Farber Cancer Institute, TU Dresden, Center for Cancer Research, German Cancer Research Center, Heidelberg University, University Hospital Heidelberg

Top 5 similar protocols

Variable analysis

independent variables

Irradiation
Erlotinib treatment
Cetuximab treatment
NU7026 (EMD Millipore, 260961) treatment
Hesperadin (Selleck, S1529) treatment
Gö6976 (LC Laboratories, G-6203) treatment
AZD6244 (Selleck, S1008) treatment
Chloroquine (Sigma, C6628) treatment
Chaetocin (Cayman, CAS 28097) treatment
Ro-32-0432 (Merck, 557525) treatment
Thymidine (Sigma, T1895) treatment

dependent variables

Cell lysates

control variables

Dimethyl sulfoxide (DMSO) used as solvent for some drugs
Double distilled water used as solvent for chloroquine
Incubation time of 1 hour for some drug treatments before irradiation or cell lysis

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!