Pulmonary Immune Response to N. brasiliensis

N. brasiliensis was maintained as described previously (14 (link)). Mice were injected subcutaneously with 750 third-stage larvae. After 9 d, mice were sacrificed and bronchoalveolar lavage (BAL) was performed using 3 mL PBS with EDTA and EGTA (0.04% each). Following red blood cell lysis in ACK, cells in BAL fluid were enumerated using the Guava Viacount assay (EMD Millipore), and leukocyte populations were quantified by cytospin and differential staining using the Hema 3 Manual Staining System (Protocol). BAL fluid was concentrated to 500 μL in Amicon Ultra 10K Centrifugal Filters (Millipore) and cytokines were measured using the mouse IL-13 and IL-9 ELISA Ready-Set-Go kits (eBioscience).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Fontana M.F., Baccarella A., Kellar D., Oniskey T.K., Terinate P., Rosenberg S.D., Huang E.J., Herbert D.R, & Kim C.C. (2015). Myeloid expression of the AP-1 transcription factor JUNB modulates outcomes of type 1 and type 2 parasitic infections. Parasite immunology, 37(9), 470-478.

Publication 2015

Guava Viacount assay Amicon Ultra 10K Centrifugal Filters

Assay Bronchoalveolar lavage Bronchoalveolar lavage fluid Cells Cytokines Edta Egta Elisa Guava Hema Il 13 Larvae Leukocyte Mouse Populations Red blood cell

Corresponding Organization :

Other organizations : University of California, San Francisco

Top 5 similar protocols

Variable analysis

independent variables

Number of third-stage larvae (750) injected subcutaneously into mice

dependent variables

Number of cells in bronchoalveolar lavage (BAL) fluid
Leukocyte populations in BAL fluid
Levels of IL-13 and IL-9 cytokines in BAL fluid

control variables

Maintenance of N. brasiliensis as described previously (reference 14)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!