Establishment of Human Myeloma Cell Lines

Human myeloma cell lines (HMCLs) LP-1 and RPMI-8226 were kindly provided by Professor Robert Orlowski (Department of Lymphoma/Myeloma, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA). JJN-3, OCI-MY5 and RPMI-8226R were kindly provided by Professor Wee Joo Chng (Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore). WL-2 was kindly provided by Professor Andrew Zannettino (Myeloma Research Programme, The University of Adelaide, Australia). KMS-11/BTZ and OPM-2/BTZ were obtained from Kyowa Hakko Kirin (Tokyo, Japan). NCI-H929 was purchased from American Type Culture Collection (Manassas, Virginia, USA). KMS-12-PE, MOLP-8, OPM-2 and U-266 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany). MMLAL29 (link) and MMKKF (unpublished) were established from the myelomatous pleural effusion of patients with myeloma. Cell cultures were maintained in RPMI-1640 medium (IMDM for LP-1, DMEM + IMDM for MMLAL), supplemented with 10% or 20% fetal bovine serum, 50 U/mL of penicillin and 50 μg/mL streptomycin, in a humidified atmosphere of 5% CO₂ at 37°C. All cell culture reagents were purchased from Invitrogen (Carlsbad, California, USA).